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Detecting cells based on menbranes with enhancement

Introduction

This guide explains you how to detect cells based on enhanced membranes using the pipeline with the Membrane Detection operation. The purpose of the pipeline is to identify cells starting from the membrane markers.

Workflow

Flowchart: ROI selection → Membrane Detection → Membrane segmenter → Export data

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Detect Cells based on Membranes with Enhancement Pipeline.
    Analysis panel: Document Pipelines list with '+ New Pipeline' and 'Detect Cells Based on Membranes...' highlighted
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window with Filter and Tags panel and a long list of Segment # entries with Volume, Volume (µm³) values 0,051–0,054 .
  2. Measurements are now visible in the objects table.
    Objects window showing table of Segment # entries and Volume, Volume (µm³) column with numeric values
  3. To show the results of each tag, click Single.
  4. For more information, refer to the Online Help (F1).

 

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