Color or RGB images are common in histopathology where bright-field images capture white light from which the diagnostic dyes absorb a certain portion of the spectrum. These images can be segmented in a range of ways, but a better separation of the signal components from the red, green, and blue channels can significantly improve segmentation results. This script is an implementation in Vision4D of the method developed by Ruifrok, A.C. & Johnston, D.A. as described here. 

This method has been developed specifically for the unmixing of diaminobenzidine, hematoxylin and eosin dies.

The output of the script is the creation of three additional channels to the current image set containing the unmixed signal.

This script relies on the NumPy libraries that are installed automatically with version 3.4 or above of arivis Vision4D. Earlier versions may require additional components.