The Spine Tracer module was introduced in arivis Pro 4.3 as a complement to the Neuron Tracer functionality introduced in arivis Vision4D 4.0. It is designed to identify and quantify neuronal spines. It requires that neurites have already been segmented in the current pipeline as a prerequisite.

The Spine Tracer is then used to detect and quantify dendritic spines in the immediate vicinity of the detected neurites.