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ZEISS Microscopy Knowledge Base

Compartmentalizing cells or particles

Introduction

The pipeline purpose is to detect objects, falling in two different classes, using Blob Finder and the Intensity Threshold operator. The detected objects are compared to find partial or total overlap conditions. It can be applied to cells, nuclei or any kind of small particles.

Workflow

ROI selection connects Blob Finder, Intensity Threshold, Segment Filter, Segment Compartmentalization, Export data.

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Application toolbar with menus File Edit View Data Navigation Objects Analysis Extras Window Help and icon buttons.
    Application toolbar row with menu labels 'File Edit View Data Navigation Objects Analysis Extras Window Help' and icon buttons
  2. In the Sample Pipelines list, double-click the Compartmentalize Cells or Particles Pipeline.
    Document Pipelines panel with '+ New Pipeline' and Sample Pipelines list showing 'Compartmentalize Cells or Particles'
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Analysis Panel button in Shortcut Toolbar is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window showing table of Segment # entries and Volume (µm³) column values like 0,051; Items: 743 .
  2. Measurements are now visible in the objects table.
    Objects window showing four parent segments (e.g. Segment #001) with Volume (µm³) values and Compartmentalization tag checked
  3. To show the results of each tag, click Single.
  4. To show the compartment details, click Master-Detail.
    Note: You must select the Compartmentalization tag first.
  5. On the top table the list of the reference objects is shown.
  6. To list the subjects belonging to them, click on a single or multiple references.
    Objects window showing filter panel with Compartmentalization checked and table listing Segment #001–#004 volumes
  7. To add or remove table columns, click Feature Columns...
    Note: Use the Master-Detail table to set the features independently for each table.
  8. For more information, refer to the Online Help (F1).
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