ZEISS Microscopy Knowledge Base
Help
ZEISS Microscopy Knowledge Base

Compartmentalizing cells or particles

Introduction

The pipeline purpose is to detect objects, falling in two different classes, using Blob Finder and the Intensity Threshold operator. The detected objects are compared to find partial or total overlap conditions. It can be applied to cells, nuclei or any kind of small particles.

Workflow

Flowchart: ROI selection → Blob Finder and Intensity Threshold → Segment Filter → Segment Compartmentalization → Export data

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Compartmentalize Cells or Particles Pipeline.
    Small menu icon showing three horizontal lines
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window with Filter and Tags panel and a long list of Segment # entries with Volume, Volume (µm³) values 0,051–0,054 .
  2. Measurements are now visible in the objects table.
    Objects window showing Type: Parents and four Segment # entries (Intensity Threshold - Nuclei) with volumes 313,613–436,822
  3. To show the results of each tag, click Single.
  4. To show the compartment details, click Master-Detail.
    Note: You must select the Compartmentalization tag first.
  5. On the top table the list of the reference objects is shown.
  6. To list the subjects belonging to them, click on a single or multiple references.
    Objects window with Filter pane and table listing Segment #001–#004 (Intensity Threshold - Nuclei) and volumes
  7. To add or remove table columns, click Feature Columns...
    Note: Use the Master-Detail table to set the features independently for each table.
  8. For more information, refer to the Online Help (F1).
Impressum
Carl-Zeiss-Strasse 22
73447 Oberkochen
Germany
Legal