ZEISS Microscopy Knowledge Base
Help
ZEISS Microscopy Knowledge Base

Detecting cells (Seeded Region Growing)

Introduction

This guide explains you how to detect cells starting from an internal seed using the pipeline with the Seeded Region Growing operation. The purpose of the pipeline is to detect objects edges starting from an internal seed. The seed is grown till it reached the main structures borders according to the defined criteria. The pipeline can be applied to Cells, Nuclei, or any kind of small particles.

Workflow

Vertical flowchart: ROI selection → Denoising → Seeded Region Growing → Export data (optional)

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Detect Cells Using Seeded Region Growing Pipeline.
    Analysis pane showing Sample Pipelines with 'Detect Cells Using Seeded Region Growing' highlighted
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Impressum
Carl-Zeiss-Strasse 22
73447 Oberkochen
Germany
Legal