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ZEISS Microscopy Knowledge Base

Detecting cells (Seeded Region Growing)

Introduction

This guide explains you how to detect cells starting from an internal seed using the pipeline with the Seeded Region Growing operation. The purpose of the pipeline is to detect objects edges starting from an internal seed. The seed is grown till it reached the main structures borders according to the defined criteria. The pipeline can be applied to Cells, Nuclei, or any kind of small particles.

Workflow

Vertical flowchart: 'ROI selection' → 'Denoising' → 'Seeded Region Growing' → 'Export data (optional)'

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Application toolbar with menus File Edit View Data Navigation Objects Analysis Extras Window Help and icon buttons.
    Application toolbar row with menu labels 'File Edit View Data Navigation Objects Analysis Extras Window Help' and icon buttons
  2. In the Sample Pipelines list, double-click the Detect Cells Using Seeded Region Growing Pipeline.
    Analysis pane with '+ New Pipeline' button and 'Detect Cells Using Seeded Region Growing' highlighted in Sample Pipelines.
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Analysis Panel button in Shortcut Toolbar is displayed. When clicking on it, the option Open How to appears.

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