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Detecting cells based on membranes

Introduction

This guide explains you how to detect cells based on membranes using the pipeline with the Membrane-based Segmenter operation. The purpose of the pipeline is to identify cells starting from the membrane markers.

Workflow

Vertical flowchart with three boxes labeled ROI selection → Membrane segmenter → Export data

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Application toolbar with menus File Edit View Data Navigation Objects Analysis Extras Window Help and icon buttons.
    Application toolbar row with menu labels 'File Edit View Data Navigation Objects Analysis Extras Window Help' and icon buttons
  2. In the Sample Pipelines list, double-click the Detect Cells based on Membranes Pipeline.
    Pane titled 'Document Pipelines' with '+ New Pipeline' button and 'Detect Cells Based on Membranes' in Sample Pipelines selected
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Analysis Panel button in Shortcut Toolbar is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window showing table of Segment # entries and Volume (µm³) column values like 0,051; Items: 743 .
  2. Measurements are now visible in the objects table.
    Objects window showing left Filter panel and a table of Segment # names and Volume, Volume (µm³) values
  3. To show the results of each tag, click Single.
  4. For more information, refer to the Online Help (F1).

 

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