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Detecting spines with neurite/neuron tracer
This article covers the Spine Tracer pipeline operation and how it can be used in conjunction with Neurite Tracer and Neuron Tracer operations.
Overview
- Start by using either the Neuron Tracer or Neurite Tracer operations to create neurites.
- Use the Spine Tracer with existing neurites to detect and quantify spines.
Introduction
The Spine Tracer module was introduced in arivis Pro 4.3 as a complement to the Neuron Tracer functionality introduced in arivis Vision4D 4.0. It is designed to identify and quantify neuronal spines. It requires that neurites have already been segmented in the current pipeline as a prerequisite.
The Spine Tracer is then used to detect and quantify dendritic spines in the immediate vicinity of the detected neurites.