1. Run the appropriate pipeline to detect the segments over time.
2. If required, apply the pre-processing steps followed by the Segmentation tools.
   
   Any segmentation operator can be used to generate the segments. Blob Finder, Watershed, Machine learning tools are available and applicable accordingly to the dataset features.
3. The segments can also be generated importing labeled images.
   
4. The segments must be detected over time. Therefore, all the time points should show the found objects.