Arivis Pro also includes background subtraction algorithms based on morphological operators. All of them are available as operators in the pipeline workflow. As mentioned in the previous chapter, the background subtraction results can be saved as a new SIS file, new Image-Set or as an additional channel in the active Image-Set for display purposes.

One of the main applications of the Methods Preserve bright/dark objects is to remove objects from an image using a structuring element that does not match the ones to be removed. The different operations produce an image in where only the removed components are present. This image is subtracted from the source one. The top-hat transformation is used for lighter objects on a dark background (white top-hat), while the bottom-hat is used for the contrary (black top-hat). The top-hat transformation effectively corrects the effects of uneven lighting (shading correction). This operation is essential when you want to carry out the segmentation.

The Morphology Filter allows to apply the White or Black Top-Hat Algorithm.

Method:

Select between the Preserve bright objects (White Top-Hat) and the Preserve dark objects (Black Top- Hat) method.

A radius value is required to define the minimum size of the objects that will be preserved by the Background subtraction.