Seeded Region Growing Parameters

Seeded Region Growing parameters

Seeding

Parameter		Description
Method		Sets the seeding method.
–	Nucleus-based	Detects the bright peaks such as nuclei.
–	Membrane-based	Detects dark peaks inside membrane staining.
Channel		Sets the analysis target channel.
Seeded Detection		Sets the seed threshold percentage. A lower value detects fewer, but bigger seeds. A higher value detects more, but smaller seeds.
Area		Sets the Area, Volume or Diameter seeds range. Only the segments that matches the range are accepted as seeds. The filter can be enabled/disabled. Note: You can measure the diameter directly from the dataset with the Measurement tool.
–	Diameter	Filters the unwanted seeds based on their diameter.
–	Volume	Filters the unwanted seeds based on their volume.
–	Area	Filters the unwanted seeds based on their area.

Parameter		Description
Method		Sets the region growing method.
–	Watershed	Segments bright objects such as cells by growing from seeds to the border of an object.
–	Membrane-based watershed	Segments dark objects such as membrane staining by growing from the seeds to the bright intensity outlines.
Channel		Sets the channel for the selected algorithm.
Threshold		Sets this parameter so that the foreground of the region growing channel contains the objects of interest.
Max. distance		Sets the maximum distance on which the region growing will act. Note: You can measure the distance directly from the dataset with the Measurement tool.