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Detecting cells or particles

Introduction

This guide explains you how to detect cells or particles using the pipeline with the Blob Finder and Feature Filter operations. The purpose of the pipeline is to detect objects having a quite limited, well defined, structure. The pipeline can be applied to cells, nuclei, or any kind of small particles.

Workflow

Vertical flowchart: rounded boxes 'ROI selection' → 'Blob Finder' → 'Segment Filter' → 'Export data' with downward arrows

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Detect Cells or Particles Pipeline.
    Analysis window showing 'Document Pipelines' header and a Sample Pipelines list with 'Detect Cells or Particles' highlighted
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window with Filter and Tags panel and a long list of Segment # entries with Volume, Volume (µm³) values 0,051–0,054 .
  2. Measurements are now visible in the objects table.
    Objects dialog showing a filter pane and a table of segments like Segment #225 (Blob Finder - Particles) with Volume column
  3. To add or remove table columns, click Feature Columns...
    For more information, refer to the Online Help (F1).

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