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Detecting nuclei and cells (Machine Learning)

Introduction

This guide explains you how to detect nuclei and cells using the pipeline with the Machine Learning Segmenter operation. Machine learning (ML) is a branch of Artificial intelligence (AI) in which, based on the training dataset that are first provided, the computer develops its own logic for answering future questions. The key concept of machine learning is to produce accurate predictions on new unseen data after being trained on a finite learning dataset.

Workflow

ML Training (dashed box) and ROI selection arrows point to Machine Learning, which points to Export data (optional)

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Detect Nuclei And Cells Using Machine Learning Pipeline.
    Analysis panel showing Document Pipelines and Detect Nuclei And Cells Using Machine Learning selected
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

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