Parameter

Description

Channel

Sets the processing and analysis target channels.

Image type

Sets the segmentation method to apply based on the image type.

–

Nucleus

This method directly uses the local maxima in the image as seeds from which to start the watershed.

–

Membrane

This method directly uses the local minima in the image as seeds from which to start the watershed.

–

Binary

This method works on binary images. It calculates a distance map for the foreground and uses the maxima in the distance map as seeds from which to start the watershed.

Threshold

Sets the threshold for segmentation. Everything above the threshold will be segmented.
Note: You can use the Picker tool to pick a threshold from the preview layer directly.

Diameter

Sets the reference objects diameter (max).
Note: Object diameter feature can be measured directly from the dataset with the Measurements tool.

Split sensitivity

Sets the sensitivity used to split touching objects.

Decreasing the Split sensitivity will fuse more objects
.
Increasing the Split sensitivity will split more objects.

Note: You can use the Calculator tool, to compute the split sensitivity.