Parameter

Description

Channels

Sets the processing and analysis target channels.

Min intensity (membrane)

Sets an approximation of a membrane with the lowest intensity. This value is used for the initial thresholding of the image to extract the membrane and thus separate it from the background. This is a very important and guiding value, therefore it needs to be selected carefully.

Split sensitivity

Sets the sensitivity used to split touching objects.

Decreasing the Split sensitivity will fuse more objects
.
Increasing the Split sensitivity will split more objects.

Membrane Maximum

Sets the membrane maximum size. Drop down the list to select the feature

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Max. diameter

Sets the value as the maximum diameter.
Note: You can measure the membrane maximum diameter directly from the dataset with the Measurement tool.

–

Max. area

Sets the value as the maximum area.
Note: You can measure the membrane maximum diameter directly from the dataset with the Measurement tool.

–

Max. volume

Sets the value as the maximum volume.
Note: You can measure the membrane maximum diameter directly from the dataset with the Measurement tool.