Detecting cells (Region Growing)

Introduction

This guide explains you how to detect cells starting from previously computed seeds using the pipeline with the Region Growing operation. The seed is grown till it reaches the main structures borders according to the defined criteria. You can apply the pipeline to cells, nuclei, or any kind of small particles.

Workflow

Flowchart of five steps: ROI selection → Denoising → Nuclei detection → Region Growing → Export data (optional)

Activating the Sample Pipeline

In the Shortcut Toolbar, click Analysis Panel .
In the Sample Pipelines list, double-click the Detect Cells Using Region Growing Pipeline.
If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Viewing the results

If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table .
Measurements are now visible in the objects table.
To add or remove table columns, click Feature Columns...
For more information, refer to the Online Help (F1).

Detecting cells (Region Growing)

Introduction

Workflow

Activating the Sample Pipeline

Viewing the results

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