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ZEISS Microscopy Knowledge Base

Detecting cells (Region Growing)

Introduction

This guide explains you how to detect cells starting from previously computed seeds using the pipeline with the Region Growing operation. The seed is grown till it reaches the main structures borders according to the defined criteria. You can apply the pipeline to cells, nuclei, or any kind of small particles.

Workflow

Flowchart: ROI selection → Denoising → Nuclei detection → Region Growing → Export data (optional)

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Application toolbar with menus File Edit View Data Navigation Objects Analysis Extras Window Help and icon buttons.
    Application toolbar row with menu labels 'File Edit View Data Navigation Objects Analysis Extras Window Help' and icon buttons
  2. In the Sample Pipelines list, double-click the Detect Cells Using Region Growing Pipeline.
    Analysis panel with 'Document Pipelines', '+ New Pipeline' button and highlighted sample 'Detect Cells Using Region Growing'
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Analysis Panel button in Shortcut Toolbar is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window showing table of Segment # entries and Volume (µm³) column values like 0,051; Items: 743 .
  2. Measurements are now visible in the objects table.
    Window titled Objects showing an objects table listing Segment #001 (Nuclei) and volume values, Items: 52
  3. To add or remove table columns, click Feature Columns...
    For more information, refer to the Online Help (F1).
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