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Tracking cells or particles

Introduction

The guide explains you how to track cells or particles using the pipeline with the Blob Finder and Tracking operation. The purpose of the pipeline is to track objects, detected using the Blob Finder operation, gathering its motion features (direction, speed, linearity, etc.). It can be applied to cells, nuclei, or any detected structures.

Workflow

Vertical flowchart: 'ROI selection' → 'Watershed' → 'Segment Tracker' → 'Export data'

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Toolbar showing menus File Edit View Data Navigation Objects Analysis Extras Window Help and tool icons.
    Analysis panel showing Document Pipelines and Sample Pipelines list with Compartmentalize Cells or Particles highlighted
  2. In the Sample Pipelines list, double-click the Track Cells or Particles Pipeline.
    Analysis window showing 'Document Pipelines', sample pipelines list with 'Track Cells or Particles' selected and + New Pipeline button
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How to guide. By hovering over a pipeline, this button Hamburger menu icon showing three horizontal lines is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window with Filter and Tags panel and a long list of Segment # entries with Volume, Volume (µm³) values 0,051–0,054 .
  2. Measurements are now visible in the objects table.
    Objects window with 'Tracking Watershed' selected and table of rows labeled 'Track #00...' and headers 'Type','Name','Volume'
  3. To add or remove table columns, click Feature Columns...
    For more information, refer to the Online Help (F1).

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