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ZEISS Microscopy Knowledge Base

Detecting dendritic spines (AI-assisted)

Introduction

The guide explains you how to trace dendritic spines using the Spine Tracer operation included in the Tracing module. The operation provides 3 methods (AI-assisted, Use Segments and Use Probability Map) to detect and connect dendritic spines to an already existing trace. This guide focuses on the AI-assisted method.

Workflow

Flowchart of boxes labeled ROI selection → Neurite Tracer → Spine Tracer → Export data (optional)

Activating the Sample Pipeline

  1. In the Shortcut Toolbar, click Analysis Panel Application toolbar with menus File Edit View Data Navigation Objects Analysis Extras Window Help and icon buttons.
    Application toolbar row with menu labels 'File Edit View Data Navigation Objects Analysis Extras Window Help' and icon buttons
  2. In the Sample Pipelines list, double-click the Detect Spines with AI assisted Method pipeline.
    Analysis panel with + New Pipeline button and Sample Pipelines list showing Detect Spines with AI-assisted Method highlighted
  3. If you have activated a pipeline, it will be replaced by the new one.

You can open the appropriate How-to guide. By hovering over a pipeline, this button Analysis Panel button in Shortcut Toolbar is displayed. When clicking on it, the option Open How to appears.

Viewing the results

  1. If not already visible, open the Objects dialog. In Shortcut Toolbar, click Objects table Objects window showing table of Segment # entries and Volume (µm³) column values like 0,051; Items: 743 .
  2. Measurements are now visible in the objects table.
    Objects dialog: left tags Neurite Tracer, Spine Tracer; right table lists Trace #001–#003 with spine counts 229,71,31
  3. To color the results more meaningfully, click Set Object Style.
  4. Click Apply to: Traces.
    Style for tag: Neuron Tracer panel with Apply to: All objects Tracks Traces (Traces selected) and Color checked
  5. To select the trace color options, select one item from the Color by list.
    Traces: Color by branch level selected; other options are Color by branch depth, Color by component, Color by structure
  6. To set further style settings, set the parameters in the Visualization section.
    Visualization section showing Opacity slider, Display buttons Cone Approximation Skeleton, and Material buttons Standard Glass

Refer to the Help (F1) for more Information.

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