As mentioned above, Volume Fusion works by taking one image set that we define as the Base image set and another that we define as the Moving image set, and transforming the Moving set to match the Base. If both the base and moving image set are similar in size and channels, which is the base and which is the moving set is relatively unimportant. However, if combining images of vastly different resolutions, as one might if combining electron microscopy and light microscopy image, it is worth bearing in mind that the moving set will be scaled to match the base set. This will usually result in the moving set being either scaled up to gigantic proportions (if it is much lower resolution) or scaled down significantly (if it is much higher resolution). Because of this, Volume Fusion is only really useful for multimodal imaging if the two imaging modes produce images of similar resolution.