Parameter

Description

Seeds

Sets the input (seeds).

Method

Sets the seeding method.

–

Watershed

Segments bright objects such as cells by growing from seeds to the border of an object.

–

Membrane-based watershed

Segments dark objects such as membrane staining by growing from the seeds to the bright intensity outlines.

Channel

Sets the analysis target channel.

Threshold

Set the region growing threshold.

Max. distance

Set the Maximum distance on which (within) the region growing will act.
Note: Use the he Measurements tool to measure the area and diameter.