Brightfield color imaging is a subtractive process where we start with white light and the dyes absorb various components of the white spectrum. The unmixing method generates images that are white with the dye reducing the signal to 0 (black/dark signal on white background). However, many segmentation methods in arivis Vision4D were implemented with fluorescence in mind as a default where the background is typically dark and the signal is a positive deviation from that (white/channel color on black background). This script has been implemented with an option to invert the output to make segmentation easier. If you want to invert the output so that the signal is bright on a dark background, you can simply change the following line:

# -----------------------------------------------------------------------------

INVERT_IMAGE = False

to 

# -----------------------------------------------------------------------------

INVERT_IMAGE = True