Calibrations are vital to make sense of the results of image analysis. They translate the units of the images, like pixels, into real world units. Calibrations in image analysis come in two forms: Spatial and Temporal. Spatial calibrations allow us to measure distances, areas and volumes. Temporal calibration allow us to measure the rate of changes in an image in absolute times. This information is normally part of the metadata of the files that are imported and is normally carried over into the SIS file during the import process. However, this metadata may on occasions be missing, inaccessible to the importer, or incorrect. In any case, it is best to check that this information is correct after import and to correct it if necessary before doing anything else with the images. Changes to calibrations are retroactively applied to measured features, but doing this prior to the analysis could save some time in having to re-export the results.