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ZEISS Microscopy Knowledge Base

Fusing Volumes

Volume Fusion is essentially a two-step process. First, we need to establish registration parameters and then we can modify the moving image set according to those parameters so that it can be fused with the base image set. The registration is used to work out translation, rotation, and scaling and can be done in either one of two ways.

Landmark registration - Creating landmarks

Landmark Registration - Fusing the volumes

Surface registration - extracting and transferring the surface

Surface registration - Transforming the surface in Vision4D

Surface registration - Transforming the surface in VisionVR

Surface registration - Applying the surface transformation for fusion

With the transformation of the surface in either Vision4D or VisionVR, we can copy the transformation parameters to the Volume Fusion. At the bottom-left of the Surface Transformation window, there are a few buttons.

Transform Surface dialog with X Y Z sliders (red/green/blue), scale and rotation fields, three circled buttons

The first two allow us to copy and paste the transformation parameters to/from the clipboard. We could use the Copy button to store the parameters and paste them into the Volume Fusion window, but it is just as easy to click the 3rd button, which opens the Volume Fusion window and copies the parameters in one click.

Volume Fusion Transformation tab with Scaling, Rotation, Translation values for X Y Z and 3D preview of overlapping boxes

Using the option to Open transformation in Volume Fusion automatically configures the input base and moving image sets, and applies the transformation parameters from our surface. If necessary we can review and change the Image Sets parameters to check that the correct sets have been selected, and for the correct handling of channels, and when ready, click Run.

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