• Optimize your segmentation as much as possible. Tracking is dependent on segmentation results and the better these are, the more accurate the tracking will be.
• Try different parameters to see which work best for your data, especially with regards to the motion types.
• Only use what you need:
• Reduce the search radius to avoid big jumps in the tracks.
• Only use weighing if the segments don't change shape/intensity much over time.
• Don't allow fusions or divisions unless this is necessary.
• Avoid manual editing tracks to reduce the time required to do the analysis and reduce user biases.
• Consider using VR for manual track editing and creation is automated 4D tracking doesn't work for your images.