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ZEISS Microscopy Knowledge Base

Tools

Import Bioformats Image Tool

This tool enables you to use Bioformats as an integrated library for reading and writing image file formats. It is capable of parsing both pixels and metadata for a large number of formats. It achieves this by converting proprietary microscopy data into an open standard called the OME data model. With Bioformats, you can read proprietary formats and convert them. For example, it is possible to load simple images you can import, such as camera images or more complex images, such as a light microscope image with overlays, to ZEN core.

Parameter

Description

File Name

The path and filename of the table to be loaded

Click on Rounded gray square icon with three centered black dots to open the file browser and select the desired file.

For more information, see ZEN Connect.

Import ZEN Connect Project Tool

Parameter

Description

Filename

The path and filename of the project to be loaded.

Click on Rounded gray square icon with three centered black dots to open the file browser and select the desired project.

For more information, see Importing a ZEN Connect Project.

For more information, see ZEN Connect Module.

ZEN Connect Tool

Within a ZEN Connect project, you manage your data in a project structure tree combined with the viewer.

Parameter

Description

2x2 grid icon of four small squaresProject / stacked layers icon showing two overlapping sheets Layers

The ZEN Connect tool provides a Layers view and a Tree view of image data that you have acquired for the ZEN Connect project. Every image that you have acquired for the ZEN Connect project is listed. As you acquire or import more image data, the new image data will be listed in the views.

Change Opacity

Adjusts the opacity of detected objects.

Align

Activates the alignment process for the selected data, see Activating the Alignment Process.

Align System

Activates the alignment process for the current microscope session, see Activating the Alignment Process.

New Session

Creates a new session.

cogwheel gear icon with central circle
Context Menu

Displays the context menu for ZEN Connect, see Context Menu.

For more information, see ZEN Connect.

ZEN Connect Renderer Tool

Parameter

Description

Tree View tool

For more information, see ZEN Connect Tool.

Color Style

Controls the color format of the export. Select RGB (color) or Intensity (black and white).

RGB Color

Based on the RGB (Red-Green-Blue) color model. Be aware that color files may be up to three times as large as intensity files.

Intensity

Images are saved in 8-bit format.

Export Format

Selects the format for the exported file.

CZI image (Single Channel)

Exports the image as a Carl Zeiss Image file. CZI files are not limited in size. The images are exported in a single channel CZI.

CZI image (Multi-Channel)

Exports the image as a Carl Zeiss Image file. CZI files are not limited in size. The images are exported as a multi-channel CZI.

Note that the resulting image might look different when reopened in ZEN than it does in ZEN Connect.

Burn in Data Bar

Activated: Burns the currently configured data bar into the exported image.

Rotation

Rotates the view to the desired orientation. Drag the slider, or in the text field, type in the value.

Pixel Size

Sets the pixel size of the export. The smaller the pixel size, the more disk space your export will take. The options Low, Medium and High only have an effect, if several images are exported as a single image.

Smallest

Sets pixel size of the finest image, i.e. the smallest pixel size.

Medium

Calculates and sets the pixel size to the log average of finest and coarsest image (i.e. 10^(0.5*(log(fine)+log(coarse)).

Largest

Sets pixel size of the coarsest image, i.e. the largest pixel size.

Custom

Sets a custom pixel size which can be entered in the input field.

Width (px)

Sets the width to directly alter the export pixel count and export area (the pixel size is unchanged).

Height (px)

Sets the height to directly alter the export pixel count and export area (the pixel size is unchanged).

Width (µm)

Displays the full width of the export area in µm.

Height (µm)

Displays the full height of the export area in µm.

Approx. Data Size

Displays an approximation for the data size of the exported image. The actual file size after export may be less, depending on compression of some file formats.

For more information, see Exporting Images from a ZEN Connect Project.

Export ZEN Connect Project (for ZEN) Tool

This tool enables you to export a ZEN Connect project file to be used in ZEN.

Parameter

Description

Destination Folder

Displays the location on the disk where you want the project file to be saved.

Click on Rounded gray square icon with three centered black dots to open the file browser and select the desired folder.

Project Name

Enter the project file name with the format <name>.aproj

For more information, see

Video Export Renderer Tool

Parameter

Description

2x2 grid icon of four small squaresProject / stacked layers icon showing two overlapping sheets Layers

 

The standard ZEN Connect Layers view/Tree view of image data that you have acquired for the ZEN Connect project. Every image that you have acquired for the ZEN Connect project is listed. As you acquire or import more image data, the new image data will be listed in the views.
Select here what data you want to export.

Show Data Bar

Activated: Burns the currently configured data bar into the exported video.

Show Region Caption

Activated: Shows the image names in the exported video.

Show Region Outline

Activated: Shows the image frames in the exported video.

Export Resolution

Sets the resolution and format for the video export.
Resolutions available in the drop-down list:

  • 320 x 240 (4:3)
  • 428 x 240 (16:9)
  • 640 x 480 (4:3) (=default value)
  • 854 x 480 (16:9)
  • 960 x 720 (4:3)
  • 1280 x 720 (16:9)
  • 1440 x 1080 (4:3)
  • 1920 x 1080 (16:9)

Zoom to Extent

Places the image to the center of the preview area.

Rotation

You can use the slider or the input field to rotate the view.

Start Delay

Sets the delay at the start of the video. The default setting is 1,0 seconds.

Key Frames

Lists the key frames, including the data of the position and FOV.

White upward-pointing chevron icon on dark square background
Move Up

Moves the selected key frame up in the list.

White downward-pointing chevron icon on dark square background
Move Down

Moves the selected key frame down in the list.

Delete icon, trash can
Delete

Deletes the selected key frame.

Go to key frame

Displays the selected key frame.

Reset key frame to current view

Sets the values of the selected key frame to the current view.

cogwheel gear icon with central circle
Options

Load Export Key Frames

Loads stored key frames.

Save Export Key Frames

Saves the key frames in XML format.

Transit to

Sets the transition time (in seconds) for zooming to a selected key frame.

Wait at

Sets the time (in seconds) to stay at the selected key frames.

Return to first at end

Activated: Returns to the first key frame at the end of the video.

Add current view as key frame

Adds the current view as a key frame.

Preview export

Displays a real-time preview of the video.

Start Export

Exports and saves the video in the folder defined in the file browser.

ROI List Tool

In this tool, you can see a list of your regions of interest (ROI) and edit or rename them.

Parameter

Description

Add ROI icon
Add ROI

Adds a region into the Image View.

List of ROI

Here you see a list of all ROI in your ZEN Connect project.
A double click on an entry moves the stage to the center of the respective ROI.

Delete icon, trash can
Delete

Deletes the currently selected region of interest.

Rename icon, pencil
Rename

Allows you to rename the currently selected region of interest.

Move to icon
Move to

Moves the stage to the center of the currently selected region.

Import Images into Session Tool

This tool enables you to import an image directly into a ZEN Connect session (e.g. as a background image).

Parameter

Description

File Names

Selects and displays the files which are imported.

Correlative Session

Selects the session where the image(s) should be imported.

Background Image

Activated: Imports the image(s) as background image.

Measurements Tool

Parameter

Description

Measurement Tools

Distance tool icon showing a horizontal line with end ticks
Distance

Allows the user to add two points to measure a distance.

Angle tool icon showing two connected line segments forming an angle
Angle

Allows the user to add three points to measure an angle.

Area tool icon showing a chevron-shaped polygon outline
Area

Allows the user to draw a polygon contour to measure an area.

Circle tool icon showing a circle with a diagonal line
Circle

Allows the user to draw a circle to measure a diameter.

Measurement table

Value

Displays the measured value.

Type

Displays the icon of the measurement type.

Visibility toggle icon showing an eye outline
Visibility

Toggles the visibility of each measurement.

Name

Displays the name of each measurement.

Delete icon, trash can
Delete

Deletes the currently selected measurement.

Create Table

Creates and opens a table document with the current measurement values.

ZEN Connect - Alignment Tool

Parameter

Description

Apply

Applies the changes for alignment.

Discard

Discards all changes for alignment.

Project/Layers

The tool provides a Layers view and a Project tree view of image data that you have in your ZEN Connect project.

Undo

Reverses the last alignment action(s).

Redo

Restores the last undone action(s).

Translate Tool

Parameter

Description

Step Size

Sets the step size for the translation in x and y.

X-Direction

Sets the translation in x direction. The reset button Circular reset button icon with a curved arrow resets the value to the default.

Y-Direction

Sets the translation in y direction. The reset button Circular reset button icon with a curved arrow resets the value to the default.

Rotate Tool

Parameter

Description

Rotation Center

Defines the rotation center around which the image is rotated. It is indicated in the Image View with a pin.

Custom

Takes the custom rotation center defined by the pin in the image.

Angle

Sets the rotation angle. The reset button Circular reset button icon with a curved arrow resets the value to the default.

Scale Tool

Parameter

Description

X-Dimension

Sets the scaling factor in x direction. The reset button Circular reset button icon with a curved arrow resets the value to the default.

Y-Dimension

Sets the scaling factor in y direction. The reset button Circular reset button icon with a curved arrow resets the value to the default.

Flip/Mirror Tool

Parameter

Description

Image mirrored horizontally
Horizontally

Mirrors the image in horizontal direction.

Image mirrored vertically
Vertically

Mirrors the image in the vertical direction.

Shear Tool

Parameter

Description

Enable

Activated: Activates the shearing mode and displays the three shearing pins in the image.

Reset Shearing Pins

Resets the pins to the default location.

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