If Smart Setup is unable to make a proposal, it is not possible to use the selected combination of dyes and contrast methods with the current microscope hardware to make acquisitions.

Select other dyes or another contrast method.

  1. Select Free Mode > 2D Multi-Channel Acquisition workbench > Channels tool > Open Smart Setup.
  2. The Smart Setup dialog opens.

    Smart Setup does not contain any dyes, contrast methods, or proposals.
  3. To add a dye or contrast method, click + Dyes & Contrast Methods.
  4. The Add Dye or Contrast Method dialog opens.
  5. From the Dye Database list, select a fluorescent dye. Use the Search field to find the desired dye quickly, and double-click to add the selected dye.
  6. From the Contrast Methods list, select the corresponding contrast method, double-click to add the selected contrast method.

    Note that you can only add channel combinations where a suitable proposal can be made based on the available hardware.
  7. Close the dialog when you have selected the desired channels.
  8. The selected dyes will be listed in the Recently Used list for your next multi-channel acquisition configuration.
  9. The proposal is displayed in the Smart Setup dialog.
  10. From the Proposals for Acquisition Parameters, select the desired parameter, see Buttons to Optimize Acquisition Parameters.
  11. Click OK.
  1. You have configured multi-channel acquisition with Smart Setup. Proceed in the 2D Multi-Channel Acquisition workbench to acquire multi-channel images. In the Channels tool, a list with the selected dyes is displayed.