The Tiles with EDF (interactive) workbench enables you to combine a tile image with extended depth of focus (EDF) acquisition. A Z-stack image is acquired for each tile.

1. The Tiles with EDF (interactive) workbench is selected.
2. The sample is sufficiently illuminated and in focus.
3. The microscope is equipped with a motorized stage.

1. Set up the camera using the Camera tool.
2. Select the desired objective in the Magnification tool.
3. Define the region of which you wish to acquire the tile image using the Tiles Setup (interactive) tool.
4. Set up the focus range and number of slices to be acquired for each tile in the Motorized Extended Depth of Focus tool.
5. Set up the stitching method to be applied after acquisition of using the Tile Stitching tool.
6. In the Workbench Area, click the Start button.

The software acquires the tiles with extended depth of focus and assembles the tile image automatically.

Each tile is acquired multiple times at different focus positions. Thus, combining tile acquisition with extended depth of focus increases the acquisition time considerably.