1. The EDF (manual focus) workbench is selected.
  2. The sample is sufficiently illuminated and in focus.
  1. Set up the camera using the Camera tool.
  2. Select the desired Mode in the Manual Extended Depth of Focus tool:
  3. Timer: Enables you to specify the acquisition interval in seconds. Choose an interval that leaves you enough time to move the stage to the next position between two acquisitions.
  4. F12 Key: Enables you to trigger the acquisition manually by pressing F12 on your computer keyboard. This leaves you as much time as you need to move the stage between two acquisitions.
  5. Stereo Microscope: Choose the desired Z-Stack Alignment method.
  6. Start the acquisition by clicking the Start button at the top of the Workbench Area.
  7. Acquire your first image.
  8. Timer: The image is acquired automatically after the specified Interval.
  9. F12 Key: Press F12 to acquire the image.
  10. Move the stage to the next Z-position.
  11. For more information see your microscope instruction manual.
  12. Acquire the next image.
  13. Continue moving the stage another step and acquiring an image over the desired focus range.
  14. Finish the acquisition by clicking Stop in the Workbench Area.

For more information on how to move the stage manually, see your microscope instruction manual.