On the light microscope

• To ensure good calibration, the objectives must be correctly calibrated.
• We recommend 20x magnification for the automatic marker detection.
• Due to potential objective shift, accuracy will be higher if you acquire images using the same objective that you used for calibration.

On the electron microscope

• The SE2 detector is well suited for marker detection.
• Switch off the beam shift and scan rotation.
• The "Stage only" setting should also be activated under Center Point or Feature.
• Use the stage backlash to increase the stage’s accuracy in locating positions.

General Points

• For images on a SEM use conductible samples.
• Initialize the stages before using Shuttle & Find.
• Calibrate again each time the holder has been inserted into a microscope.
• If the marker is a little dirty, it can be helpful for detection purposes to de-focus it slightly.
• In the case of stitched tiles images accuracy is reduced.