Defining a Lightfield 4D Multi-Position Experiment with the Tiles Advanced Setup
- You have set up one or more Lightfield 4D tracks, see Setting Up a Lightfield 4D Track without Smart Setup or Setting Up a Lightfield 4D Track with Smart Setup.
- On the Acquisition tab, activate Tiles.
- The Tiles tool is available in the Multidimensional Acquisition section.
- Open the Tiles tool and click Show Viewer.
- The Tiles Advanced Setup view opens, see Tiles Advanced Setup. Note that the functionality of the advanced setup for Lightfield 4D experiments is limited to position experiments.
- To check the sample at the current stage position, click Live to start a live acquisition.
- In case of a live acquisition in the Tiles Advanced Setup, the live image opens as a separate image container and is also displayed at the stage position in the setup (blue rectangle).
- The Lightfield 4D tab with the view options for the acquisition is only available if you select the Live image container. To display view option tabs for both containers, go to the document bar on the right of the image containers, click
and select Separate View Controls.
- Click Stop.
- The live image acquisition stops.
- Use the left and top toolbar of the view to define your positions. For detailed information, see Tiles & Positions with Advanced Setup.
- You have set up your positions. They are displayed in the view as well as the table of the Tiles tool.
- To change z-values of your positions or verify them, see Adjusting Z-Values of Positions.
- The z-values are changed or verified.
- Start your experiment by clicking Start Experiment, or continue setting up other parameters for your Lightfield 4D experiment, see also Setting Up and Starting a Lightfield 4D Experiment.
- You have defined multiple positions for your experiment.