1. You have set up a tile experiment with at least one position.
2. You are on the Acquisition tab in the Tiles tool.

1. To check and adjust the z-value of positions, open the Positions section.
2. The z-values are displayed in the Z column of the Single Positions list.
2. Double-click on the list entry of the position that you want to check.
3. The stage automatically locates the position.
3. Use the Live mode to check the z-position of the position. To adjust the z-value, set the desired position using the focus drive.
4. Select a position in the Single Positions list, click and select Set Current Z For Selected Positions. Alternatively, in the Single Positions list, right-click the position entry and select Set Current Z For Selected Positions.
5. The current z-value is set for the respective position.
5. To check and adjust a large number of positions, use the Verify Positions dialog. Click Verify Positions in the Positions section.
6. The Verify Positions dialog opens.
6. Select the Helper Method you want to use. This will support you in determining the z-values. The options are Autofocus (AF) and Definite Focus (DF). If you have neither then you can only adjust z-values manually.
7. Click Move to Current Point. Alternatively, you can double-click on the position in the list that you want to check.
8. The stage moves automatically to the position in the list that is highlighted in blue.
8. In the Live mode use the Focus (or SW Autofocus) tool to adjust the desired z-value.
9. Click Set Z and Move to Next.
10. The position is marked with a check mark.
11. The stage moves automatically to the next position in the list.
10. Repeat the last 3 steps until you have checked all the points in the list.
11. The message All points have been verified appears.
11. Close the Verify Positions dialog.

1. You have successfully verified and adjusted the individual z-values for positions.