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Adjusting Z-Values

If you add positions or tile regions, the current Z-value is automatically adopted for the tile region or position.

To ensure that the individual z values of the tile regions and/or positions are taken into account, ZEN automatically selects the most appropriate focus strategy when the checkbox Tiles is activated. For the experiment described here no further modification needs to be made. If you want to acquire all tile regions at the same z-position, then you must select None from the dropdown list in the Focus Strategy tool. The individual z-positions are then ignored and the current z-position at the time the experiment is started is used for all tile regions. In the case that you do not have the Tiles & Positions module, ZEN will still select the appropriate focus strategy for you.

Adjusting Z-Values of Tile Regions

  1. You have set up a Tiles experiment with at least one tile region.
  1. To check the z-value of tile regions, open the Tile Regions section in the Tiles tool.
  2. The z-values of the tile regions are displayed in the Z column of the list.
  3. Double-click on the list entry of the tile region that you want to check.
  4. The stage automatically locates the center of the tile region and the associated z-position.
  5. Use the Live mode to check the z-value of the tile region.
  6. To adjust the z-value, set the new z-position with the Focus tool.
  7. In the Tile Regions list, click on Options and select Set Current Z For Selected Tile Regions. Alternatively, in the Tile Regions list, right-click the tile region entry and select Set Current Z For Selected Tile Regions.
  8. To check further tile regions, repeat steps 2 to 4.
  9. To check and adjust large number of tile regions, click on the Verify button.
  10. The Verify Tile Region dialog opens. There you have an interface for the verification process of each tile region.
  11. Click on Close after you have verified all tile regions.
  1. You have successfully checked and adjusted the individual z-values for the tile regions.

Adjusting Z-Values of Positions

  1. You have set up a tile experiment with at least one position.
  2. You are on the Acquisition tab in the Tiles tool.
  1. To check and adjust the z-value of positions, open the Positions section.
  2. The z-values are displayed in the Z column of the Single Positions list.
  3. Double-click on the list entry of the position that you want to check.
  4. The stage automatically locates the position.
  5. Use the Live mode to check the z-position of the position. To adjust the z-value, set the desired position using the focus drive.
  6. Select a position in the Single Positions list, click and select Set Current Z For Selected Positions. Alternatively, in the Single Positions list, right-click the position entry and select Set Current Z For Selected Positions.
  7. The current z-value is set for the respective position.
  8. To check and adjust a large number of positions, use the Verify Positions dialog. Click Verify Positions in the Positions section.
  9. The Verify Positions dialog opens.
  10. Select the Helper Method you want to use. This will support you in determining the z-values. The options are Autofocus (AF) and Definite Focus (DF). If you have neither then you can only adjust z-values manually.
  11. Click Move to Current Point. Alternatively, you can double-click on the position in the list that you want to check.
  12. The stage moves automatically to the position in the list that is highlighted in blue.
  13. In the Live mode use the Focus (or SW Autofocus) tool to adjust the desired z-value.
  14. Click Set Z and Move to Next.
  15. The position is marked with a check mark.
  16. The stage moves automatically to the next position in the list.
  17. Repeat the last 3 steps until you have checked all the points in the list.
  18. The message All points have been verified appears.
  19. Close the Verify Positions dialog.
  1. You have successfully verified and adjusted the individual z-values for positions.

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