This module enables you to create overview images of large areas of your sample.
For the interactive panorama acquisition following prerequisites are necessary:
Before starting the experiment itself, we recommend tthat you first take a reference image for the shading correction. This image will be used later for processing the panorama image.
Ensure that the field of view is absolutely free of any structures (dirt); as this would cause errors in the images acquired later. If necessary, slightly de-focus the sample to suppress these structures.
The field of view of your microscope might be too small for the sample area you wish to acquire. You can automatically visualize panorama images from a sample area which is larger than the camera sensor can cover by means of a single snap.
With the Live Panorama tool, you can move the stage while the software automatically acquires individual images, stitches them together and creates a panorama image.
Note that Live Panorama works for un-coded and un-motorized stages as well as motorized stages.
The next chapters will show you how to process panorama images with the Stitching processing function. Using this method you can correct an offset between the tile images. We will show you the different settings and make a comparison of the output images. So you can see which settings will give you the best result.
If your tile images contain a certain background shading, you can correct this if you have acquired a reference image for the shading correction. This image has to be opened in the Center Screen Area.
If you do not have any image available for the shading correction, you alternatively can calculate a background image from the input image via the second selection Automatic under Correct Shading. This will only work, if your input image does not include large dark regions.
You can create a Multi Image to compare the different results of your processed images via the Splitter-Mode.
Here you can adjust settings for the panorama acquisition.
After you have acquired the single tile images simply activate the Perform stitching after the experiment checkbox. If you want to stitch the images manually, click on the Perform Stitching button, after you have finished the experiment.
The panorama acquisition itself is performed interactively via in the Panorama view. If you click on Start Experiment' the Panorama view opens and you can start to acquire the single tile images.
In this view you see the representation of the microscope stage. The Live image from the camera (blue frame) is automatically shown in the middle of the image area. Furthermore a tool window is displayed, that allows you to control the image acquisition, e.g. perform auto exposure or acquire an individual tile image.
The tool window for panorama view is normally visible in the lower right corner of the center screen area. It becomes active if you move the cursor over it. You can use it to set acquisition parameters and acquire tile images for your panorama image.
|
Parameter |
Description |
|---|---|
|
Center to Live Navigator |
Centers the stage view at the current position of the Navigator frame. |
|
Action Buttons |
With the three action buttons (Live, Set Exposure, Continuous) you're able to control acquisition parameters like you are used to do it on the Acquisition tab. |
|
Acquire Tile Image |
Acquires a tile image. This comprises all activated channels as well as Z-stacks. After the acquisition, the tile image is placed in the corresponding location in the stage view. |