Compatible Objectives

Lightfield 4D imaging is only available for certain compatible objectives. A message in the Experiment Manager section of the Acquisition tab (below Start Experiment) indicates if the currently selected objective is compatible with Lightfield 4D acquisition. In case it is not, use the Microscope tool in the Right Tool Area to change your objective.

Overwriting Settlings

All previously made channel and light path settings are overwritten by Smart Setup.

  1. On the Acquisition tab, click Smart Setup.
  2. The Smart Setup dialog opens. The LF4D mode is available if the correct hardware and license are available.
  3. Click LF4D.
  4. You are in Lightfield 4D setup mode.
  5. Click + Dye & Contrast Methods.
  6. The dye selection dialog opens.
  7. Double click the dyes you want to use and close the dialog.
  8. The dyes are added to the table and the Smart Setup displays Proposals for Specific Filter Settings.
  9. Select the proposal you want to use and click OK.
  10. The Smart Setup closes, a channel is created for every dye, and the light path is set up correctly.
  11. Time Series is activated and the Time Series tool opens.
  12. If you have selected the proposal for fastest acquisition, Acquire with High Speed is automatically activated if all channels are using the same filter position in the reflector changer. Channels requiring different filter sets cannot be combined in a high-speed acquisition. If you have defined two or more Lightfield 4D tracks for multi-channel experiments, the exposure time for each channel is identical. Updating the exposure time of one channel updates it for all defined Lightfield 4D channels in a high-speed acquisition.
  1. You have set up a Lightfield 4D track. You can now configure the rest of your experiment and start it.
  2. All dimensions and functionalities that are not suitable for Lightfield 4D experiments (like Experiment Feedback) are deactivated after setting up the track.