No Combination with other Tracks
Note that Lightfield 4D tracks do not work in combination with other track types, for example confocal tracks. If you have active Lightfield 4D tracks, other tracks are set inactive and vice versa.
Compatible Objectives
Lightfield 4D imaging is only available for certain compatible objectives. A message in the Experiment Manager section of the Acquisition tab (below Start Experiment) indicates if the currently selected objective is compatible with Lightfield 4D acquisition. In case it is not, use the Microscope tool in the Right Tool Area to change your objective.
- On the Acquisition tab, open the Imaging Setup tool, or the Channels tool.
- The track selection is displayed.
- Select Lightfield 4D.
- The dye selection dialog opens.
- Double click the dyes you want to use and close the dialog.
- The Lightfield 4D track is configured with an exposure time of 50 milliseconds.
- Time Series is activated and the Time Series tool opens.
- Acquire with High Speed is automatically activated if all channels are using the same filter position in the reflector changer. Channels requiring different filter sets cannot be combined in a high-speed acquisition. If you have defined two or more Lightfield 4D tracks for multi-channel experiments, the exposure time for each channel is set the same. Updating the exposure time of one channel updates it for all defined Lightfield 4D channels.
- You have set up a Lightfield 4D track. You can now configure the rest of your experiment and start it.
- Dimensions and functionalities that are not suitable for Lightfield 4D experiments (like Experiment Feedback) are deactivated after setting up the track.