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Operating the Image Analysis Wizard

Defining Measurement Classes

This step in the Image Analysis Wizard enables you to create measurement classes for the sample. A class corresponds to a type of object to be detected, for example objects with a specific brightness or shape.

This step enables you to define how many measurement classes are required. The properties of a class (i.e. how an object is detected) are defined in a later step. When you create a class it is added to the list of classes. The class itself contains the measurement values for each individual detected object. To define the classes for the image analysis:

  1. Click Add Class.
  2. The new class is always added below the currently selected item.
  3. Enter a meaningful name for the class.
  4. In a multichannel image, select which channel should be evaluated.
  5. Select the color in which the detected objects should be displayed.
  6. Click Next.

Specifying Region of Image to be Analyzed (Frame)

This step in the Image Analysis Wizard enables you to specify the following:

  • The areas of the image to be measured (measurement frames)
  • How objects at the edge of the image or frame are treated
  1. If you wish a user to be able to adjust the settings in this step when the job is run, activate Interactive.
  2. Specify the measurement area:
  3. Use the drawing tools to create one or more new areas.
  4. You can modify measurement areas by dragging them in the image or entering coordinates.
  5. If you wish to measure the entire image, click the icon to delete all measurement areas.
  6. In Mode, specify how objects at the edge of the image or the measurement area are treated.
  7. Click Next.

For detailed information about each parameter, see Frame.

Automatically Correcting Object Detection (Condition)

This step in the Image Analysis Wizard enables you to specify which of the detected objects are measured, based on various properties such as area, intensity, or roundness.

All the objects detected with the current settings are highlighted in the image.

You can manually add or remove objects that are difficult to detect in the next step.

  1. If you wish this step to be included when the job is run, activate Execute.
  2. Otherwise the step is skipped.
  3. If you wish a user to be able to adjust the settings in this step when the job is run, activate Interactive.
  4. Select the class for which you wish to modify the settings.
  5. You can specify different settings for each class.
  6. Click Edit.
  7. Double-click the properties you wish to use to select detected objects.
  8. The selected properties are added to a so-called block. The properties are joined by "And" conditions: only detected objects which fulfill all the properties in a block are measured; all the other detected objects are excluded.
  9. You can filter the list by entering the first few letters of the property name in Search Feature or by selecting the corresponding category of property in the drop-down.
  10. For more information about the properties and their values, see Feature Selection Dialog.
  11. To create an alternative block, click Add Block.
  12. The blocks are joined by an "Or" condition. Objects that fulfill all the conditions in at least one block are measured.
  13. Double-click the properties to add to the new block.
  14. Repeat steps 5-7 as desired.
  15. When you have selected all the desired properties, click OK.
  16. For each property, click a representative object in the image.
  17. The threshold values for the property are set based on the values of the selected object.
  18. If desired, correct the lower and upper threshold values in Minimum and Maximum respectively.
  19. If the object's property is not between the thresholds, it is excluded.
  20. If you do not want to include a threshold for a property, deactivate the corresponding checkbox. In this case, the threshold is set to the minimum or maximum possible value respectively.
  21. Click Next.

For detailed information about each parameter, see Region Filter.

Manually Correcting Object Detection (Interactive Segmentation)

This step in the Image Analysis Wizard enables you to add or remove individual objects to be measured. The step can be considered a manual "fine-tuning" of the automatic detection results of the previous steps. After this step, all the objects you wish to measure should be selected and any you do not wish to be measured should be removed.

All the objects detected with the current settings are highlighted in the image.

If you need to make large numbers of manual corrections, it might be quicker to try adjusting the parameters in the previous steps first.

  1. If you wish this step to be included when the job is run, activate Interactive.
  2. Otherwise the step is skipped.
  3. Select the class for which you wish to modify the settings.
  4. You can specify different settings for each class.
  5. To add a new object or remove part an object, click Draw or Erase and use a drawing tool in the image.
  6. Draw: Adds the area drawn as a new object
  7. Erase: Removes the area drawn from any existing objects; the remaining parts of the objects are measured
  8. For more information about how to use the drawing tools, see Interactive Segmentation.
  9. To split an existing object into multiple objects click Cut and draw a line in the image where the object should be split.
  10. The resulting objects are measured separately.
  11. To extend the size of an object click Merge and use a drawing tool in the image.
  12. If the area drawn overlaps an existing object, the area and object are joined into a single object.
  13. To fill an object containing a hole, click Fill then click an object in the image.
  14. The hole is filled and the complete object is measured.
  15. To delete objects, click the desired action and then click an object in the image:
  16. Remove: Deletes the selected object; the object is no longer measured
  17. : Removes all objects; no objects are measured
  18. To expand or reduce the size of an object based on the brightness of surrounding pixels, click + or - and click a representative area in the image. The amount by which the object expands or reduces depends on the brightness of the selected pixel, its proximity to other objects, and the values of the Intensity and Color parameters.
  19. +: Expands the closest object
  20. -: Reduces the closest object
  21. Click Next.

For detailed information about each parameter, see Interactive Segmentation.

Creating Custom Features

  1. You are in the Features step of the analysis wizard in Free Mode or the respective step in Job Mode.
  1. Select a class in the list and click Custom Feature.
  2. The Custom Feature Editor opens. All already defined features are displayed in the list, or on initial opening an empty default entry is already created.
  3. In the Custom Features list, click + to add a new entry. Alternatively, if no feature has been defined yet, select the automatically displayed default entry.
  4. A new entry is added to the list.
  5. Under Define Custom Feature, define the Name for your feature and optionally specify a Unit, if applicable.
  6. In the Define Operands list, click + to add a new operand. Alternatively, if no operand has been defined yet, select the automatically displayed default entry.
  7. A new operand entry is created.
  8. Select the Class which is used to generate the operand.
  9. In the Features dropdown list, select the measurement feature that you want to use to define the operand.
  10. The selected class and measurement feature are displayed as Expression.
  11. Repeat the previous steps to define all operands you need to calculate your custom feature.
  12. All defined operands are displayed in the Define Operands list.
  13. Under Define Custom Expression, enter your operands and use the mathematical operators to define the calculation for your custom feature, e.g. 100*(a/b+Math.Pow(c,2)).
  14. Click Verify Expression.
  15. The syntax of your expression is checked and verified. In case the expression is not valid, an error message is displayed.
  16. Repeat this whole workflow to create all custom features required for your image analysis.
  17. All created features are displayed in the Custom Features list of the respective class.
  18. Click OK.
  19. The editor closes and saves the defined custom features. They are displayed in the list of the Features step of the wizard.
  20. After analyzing an image with the setting, the custom features are displayed in the result table of the respective class.

Creating Custom Statistical Features

  1. You are in the Statistics step of the analysis.
  1. Select a class in the list and click Define Custom Feature.
  2. The Custom Feature Editor opens. All already defined features are displayed in the list, or on initial opening an empty default entry is already created.
  3. In the Custom Features list, click + to add a new entry. Alternatively, if no feature has been defined yet, select the automatically displayed default entry.
  4. A new entry is added to the list.
  5. Under Define Custom Feature, define the Name for your feature and optionally specify a Unit.
  6. In the Define Operands list, click + to add a new operand. Alternatively, if no operand has been defined yet, select the automatically displayed default entry.
  7. A new operand entry is created.
  8. Select the Class which is used to generate the operand.
  9. In the Features dropdown list, select the measurement feature that you want to use to define the operand.
  10. Select the Statistical Operation the operand is used for.
  11. The selected class and measurement feature are displayed as Expression.
  12. Repeat the previous steps to define all operands you need to calculate your custom feature.
  13. All defined operands are displayed in the Define Operands list.
  14. Under Define Custom Expression, enter your operands and use the mathematical operators to define the calculation for your custom feature, e.g. 100*(a/b+Math.Pow(c,2)).
  15. Click Verify Expression.
  16. The syntax of your expression is checked and verified. In case the expression is not valid, an error message is displayed.
  17. Repeat this whole workflow to create all custom features required for your image analysis.
  18. All created features are displayed in the Custom Statistic Features list.
  19. Click OK.
  20. The editor closes and saves the defined custom statistical features. They are displayed in the list of the Statistics step of the wizard.
  21. After analyzing an image with the setting, the statistical features are displayed in a result table.

Previewing Measurement (Measure)

This step in the Image Analysis Wizard displays a preview of the measurement results of the selected class. These results are calculated roughly and may differ from the actual results when the image analysis is performed.

  1. Check the measurement results for each class.
  2. If you are not satisfied with the results, change the settings in the previous steps.
  3. Click Finish to save the image analysis.

For detailed information about each parameter, see Results Preview.

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