The following instruction helps setting up the tiles region acquisition with the Axiocam 705 pol using both cameras.
The dual-camera support for TCA is approved for the following combination of digital cameras: Axiocam 705 pol and Axiocam 305 color or Axiocam 705 color.
The image orientation must be harmonized for both cameras to ensure the correct setting for tiles region acquisition and the correct lateral alignment of the particles. The settings are dependent on the microscope hardware and the MTB configuration of the stage.
- Attach the Axiocam 705 pol on top and the Axiocam 305 color / 705 color on the side of the dual adapter.
- Add both digital cameras to one MTB configuration.
- Rotate the Axiocam 705 pol and the digital color camera on the corresponding C-mount adapter to achieve for each a parallel camera-to-stage orientation.
- Select the Axiocam 705 pol in Maintenance > Select Active Camera or via the Select Camera tool in any of the acquisition workbenches.
- Define in Free Mode a manual scaling for Axiocam 705 pol, for all objectives and if zoom objectives are available, in addition for a minimum of 5 zoom magnification levels.
- Select, if required, the correct image rotation for Axiocam 705 pol in 2D-Acquisition > Extended Camera > Model Specific > General > Orientation for correct tiles region acquisition without stitching.
- For the validation define a tiles region with the large cross in the center of the particle test slide and acquire a small tiles region to verify correct tiles image acquisition without stitching.
- Select the digital color camera in Maintenance > Select Active Camera or via the Select Camera tool in any of the acquisition workbenches.
- Define a manual scaling for the digital color camera, for all objectives and if zoom objectives are available, in addition for a minimum of 5 zoom magnification levels.
- Adjust the image rotation for the digital color camera in 2D-Acquisition > Extended Camera > Model Specific > General > Orientation so that in camera live mode a centered particle or object has the same orientation independent which camera is selected.
- To align the zoom factor for Axiocam 705 color and Axiocam 705 pol, change the default setting for Axiocam 705 color in Extended Camera > Mode section > Live Speed drop down list to High Res.