This module enables you to acquire an overview image (or panorama image) exceeding the image size of a single image. This is the case when the field of view of your microscope is too small for the sample area you wish to acquire. Acquire a set of connected, overlapping images (tiles) manually to stitch the tiles together to a single large image.
Panorama
Acquiring a Panorama Image (automatic)
Using the Panorama (automatic) workbench you can automatically acquire panorama images from a sample area which is larger than the camera sensor can cover by a single snap. By moving the stage the software automatically acquires and stitches the individual images and creates a panorama image. Note that automatic panorama works also for coded and manual stages.
- You have set-up and configured your microscope system correctly.
- You have started the software and activated the module.
- You are in Free Mode.
- In the Panorama (automatic) workbench, click Live to get a live image from the microscope camera. Adjust the camera and microscope settings to see a well illuminated and sharp live image.
- Navigate to a specific area on your sample you want to image and click Start.
- After a short moment, the camera rectangle changes to green and you can move the stage. Note that when the software loses the stitching algorithm, the color of the rectangle changes to orange or red. Then you have to manually go back to the last "good" position.
- The panorama acquisition starts. You see the live image of the sensor area.
- Move the stage slowly by using the hardware or software joystick, e.g. in right direction.
- During the stage movement the software automatically acquires the panorama image.
- In our example we now move the stage a bit up and back to the starting point.
- To finish the acquisition, click Stop.
- The panorama image will be added to the Documents Area. Save the final image on your file system.
Panorama (automatic) Workbench
This workbench enables you to acquire panorama images from a sample area which is larger than the camera`s sensor can cover by a single snap. By moving the stage the software automatically acquires and stitches the individual images and creates a panorama image. Note that automatic panorama works also for coded and manual stages.
To learn how to acquire panorama images automatically, see Acquiring a Panorama Image (automatic).
See also
Panorama Acquisition Options Tool
This tool can only be added to the Panorama (automatic) workbench. Note that the options should only be activated, if you have problems with the camera/live image during acquisition. It is only available for troubleshooting up and normally not needed.
The default state of the checkboxes is set to "undefined" (checkboxes are marked with a rectangle) which means the software tries to use an determined value for the current camera automatically. Meaning if you are unsure, if your camera supports the functionality, we recommend to leave the state to "undefined".
|
Parameter |
Description |
|---|---|
|
Use Camera Streaming (if possible) |
If activated, the software uses the free running/streaming mode of the camera. This option is intended for slower cameras. |
|
Stop and Go |
If activated, during acquisition you have to move the stage, e.g. about one camera frame, and the stop the movement. Then you have to move the stage for the next frame and stop again. Compared to the "default" mode, the images to be stitched are acquired only when the stage movement stops. This option is intended for cameras that produce motion artefacts (e.g. rolling shutter effect) and for exposure times greater than about 50 ms. |