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Smart Setup Dialog

1

+ Dye & Contrast Methods

Opens the Add Dye or Contrasting Method dialog, see Configuring Multi-Channel Acquisition with Smart Setup.

2

Table with selected channels

You can add up to 8 fluorescence channels and contrast methods. The added dyes or the contrast methods are shown in the list.

If necessary, change the color by clicking the arrow and select a user defined color.

3

Buttons to optimize Acquisition Parameters

Click on a button to optimize image acquisition regarding particular requirements and to influence parameters like the camera, detector, and lighting settings, see Buttons to Optimize Acquisition Parameters.

4

Proposals for Specific Filter Settings

Displays various Smart Setup proposals for further experiment settings.

See Graphical Display of Proposals and Smart Setup Proposals.

5

Show Excitation checkbox

Activated: Shows the excitation spectrum of the selected dyes in the graphical display.

6

Show Emission checkbox

Activated: Shows the emission spectrum of the selected dyes in the graphical display.

Buttons to Optimize Acquisition Parameters

The automatic settings you make through these buttons affect the settings displayed in the Channels tool, which are used for acquisition, see Channels Tool.

Parameter

Description

Automatic
  • Sets power of Colibri-LEDs depending on wavelength and power of transmitted and reflected light sources to 10% or 3V.
  • The system tries to set the optimal resolution for the camera by adjusting the binning if supported. The resolution is calculated from the total magnification at the camera adapter (objective + optovar + adapter).
  • Sets the Exposure Time:
    • Transmitted light channel: 10 ms
    • Reflected light channel: 10 ms
    • Fluorescence channel: 150 ms
  • Sets the Auto Exposure to Off
  • Sets Intensity to the following:
    • Transmitted light channel: 70%
    • Reflected light channel: 70%
    • Fluorescence channel: 30%

Speed
  • Sets power of Colibri-LEDs 100% and power of transmitted and reflected light sources to 10% or 3V.
  • The system tries to set the optimal resolution for the camera by adjusting the binning if supported. The resolution is calculated from the total magnification at the camera adapter (objective + optovar + adapter).
  • Sets the Exposure Time:
    • Transmitted light channel: 3 ms
    • Reflected light channel: 3 ms
    • Fluorescence channel: 50 ms
  • Sets the Auto Exposure to Off
  • Sets the Intensity to the following:
    • Transmitted light channel: 30%
    • Reflected light channel: 30%
    • Fluorescence channel: 15%

Signal
  • Sets power of Colibri-LEDs 75% and power of transmitted and reflected light sources to 10% or 3V.
  • The system tries to set the optimal resolution for the camera by adjusting the binning if supported. The resolution is calculated from the total magnification at the camera adapter (objective + optovar + adapter).
  • Sets the Exposure Time:
    • Transmitted light channel: 20 ms
    • Reflected light channel: 20 ms
    • Fluorescence channel: 300 ms
  • Sets the Auto Exposure to Off
  • Sets the Intensity to the following:
    • Transmitted light channel: 100%
    • Reflected light channel: 100%
    • Fluorescence channel: 60%

Default
  • Sets power of Colibri-LEDs 10% and power of transmitted and reflected light sources to 10% or 3V.
  • The system tries to set the optimal resolution for the camera by adjusting the binning if supported. The resolution is calculated from the total magnification at the camera adapter (objective + optovar + adapter).
  • Sets the Exposure Time to 20ms.
  • Sets the Auto Exposure to Default
  • Sets the Intensity to 100%.

Current

NOTICE! If you changed hardware settings manually and do not want to lose them, click Current.

  • Sets power of Colibri-LEDs depending on wavelength and power of transmitted and reflected light sources to 10% or 3V.
  • The system tries to set the optimal resolution for the camera by adjusting the binning if supported. The resolution is calculated from the total magnification at the camera adapter (objective + optovar + adapter).
  • Transfers the following values from the current camera in use to the Channels tool:
    • Auto Exposure
    • Exposure Time
    • Intensity

Smart Setup Proposals

The number and type of proposals depend on the used microscope hardware, the selected dyes, and the contrast method.

Parameter

Description

Best Signal

Results in the best signal strength.

Fastest

Results in the fastest acquisition.

Best Compromise

Results in the best compromise between signal strength and acquisition speed.

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