The bars in the graphs only show relative values. The actual strength of the emission signal and the crosstalk in the image can deviate substantially from this estimate, as Smart Setup has no knowledge of the strength with which the sample has been dyed with the individual dye components.

Emission Signal, Speed, Crosstalk, and Tracks

1

Emission Signal
A filled, colored bar in the Emission Signal display field shows the relative emission signal to be expected for the corresponding channel. The channel color corresponds to the color of the selected dye in the Configure Experiment section.

2

Speed
A gray bar in the Speed display field represents the approximate acquisition speed that can be expected. This is the time required for the movement of microscope hardware during multichannel acquisition. Camera exposure times or parameters for other acquisition dimensions are not taken into account here.

3

Crosstalk
A hatched bar in the Crosstalk display field shows the expected relative crosstalk originating from one or more dyes for other channels.

4

Tracks display

Only visible if the Show Excitation and/or Show Emission checkboxes are activated.

The various tracks are labeled with T1, T2 etc. The white lines show the excitation and emission spectra of the dyes schematically. The spectra are filled in color in the places that will be acquired by the acquisition configuration suggested by Smart Setup. Transmitted light channels are displayed as a white field.