This view options tab is only visible if you have opened a Lightfield 4D image document.

Parameter

Description

Raw Data

Displays the raw data image of the selected single lens.

Selected Lens

Only visible if Raw Data is selected.
Selects the respective microlens of the Lightfield 4D multi-lens array.

Volume Definition

Only visible during live acquisition.
Activates the volume definition mode with a color-coded display of different regions of the image.

Volume Color Coding

Only visible if Volume Definition is selected.
Displays the colors for different z-areas in the image to find the center of your sample volume, see Lightfield 4D Volume Color Coding.

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Top

Displays the color for image areas that are above focus.

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Center

Displays the color for image areas that are in focus.

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Bottom

Displays the color for image areas that are below focus.

Lightfield 4D

Only visible for fully acquired Lightfield 4D images and not during a live acquisition.
Displays the image with Lightfield 4D processing applied and displays the respective parameters for processing below. The Lightfield 4D reconstruction algorithm consists of two steps, a rolling ball background subtraction and Richardson-Lucy deconvolution with total variation regularization.

Iterations

Sets the number of iterations of a (constrained iterative) deconvolution. A higher value results in sharper objects but will also amplify noise and measurement errors. The available value range is between 5 and 50 iterations.

Background Correction

Sets the rolling ball radius to remove homogeneous background for better reconstruction. Larger values remove bigger structures, while smaller values also remove smaller structures and have a stronger effect on the image. The available value range is between 75 and 5 pixels.

Total Variation Denoising

Reduces negative effects of noise on the deconvolution by smoothing homogenous areas while preserving edges (sharp contrasts). The available value range is between 0 and 100%.

Parameter Configuration

 

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Store

Stores the current parameter values.

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Recall

Recalls parameter values from storage, if available.

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Reset

Resets all parameters to factory defaults.

Process Image

Selects the image dimensions, for example Current Timepoint, to create a processed image with the currently selected parameter values.

Create Processed Image

Creates an image processed with the current parameter values. The processing always takes the currently displayed image. If you have an image of a multi-position experiment and deactivate the Scene checkbox on the Dimensions tab, all positions are displayed in the Image View and processed.

The image is saved at the location set in the Lightfield 4D Processing function, see Lightfield 4D Processing. To change the path in this tab, you have to change the storage location in the processing function and execute the function once on the Processing tab.