This method enables you to process Lightfield 4D data. The Lightfield 4D reconstruction algorithm consists of two general steps, a rolling ball background subtraction and Richardson-Lucy deconvolution with total variation regularization.

Parameter

Description

Storage Location

Displays the location where the processed file is saved. Click to open a file explorer to change the location. Note that also processed images created with the Lightfield 4D tab below the image are saved at this location.

Adjust per Channel

Only available for images with multiple channels.
Activated: Opens a list with the channels to allow an individual adjustment of the parameters for each channel. For every channel you have the following options:

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Process Channel

Processes this channel according to the set parameters.

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Skip Channel

Skips this channel when processing. This channel will not be in the output image.

Iterations

Sets the number of iterations of a (constrained iterative) deconvolution. A higher value results in sharper objects but will also amplify noise and measurement errors. The available value range is between 5 and 50 iterations.

Background Correction

Sets the rolling ball radius to remove homogeneous background for better reconstruction. Larger values remove bigger structures, while smaller values also remove smaller structures and have a stronger effect on the image. The available value range is between 75 and 5 pixels.

Total Variation Denoising

Reduces negative effects of noise on the deconvolution by smoothing homogenous areas while preserving edges (sharp contrasts). The available value range is between 0 and 100%.