This view displays your experiment and the diffusion data of the individual measurement positions. For information about calculations, see Calculation of Concentration and Diffusion Coefficient. The different areas containing the image, the charts and the table can be resized by dragging the individual area boundaries with the pressed left mouse button.
1
Preview Image
Here you can see the acquired preview image with the measurement positions marked in the view.
2
Parameter Table
This table displays the data for the different measurement positions. Each position is displayed as a separate row. For details, see Diffusion Parameter Table.
3
Polar Heatmap
This diagram area gives you the visual information about the diffusion in your sample and which Airyscan fibers are used for the correlation, see Polar Heatmap. A right click into the area enables you to export the polar heatmap as an image.
4
Correlation Chart
Here you have the correlation chart for the currently selected spot. You can zoom into the displayed curve with the mouse wheel or by drawing a zoom rectangle with pressed left mouse button. A right click in the chart area resets the chart (zoom) to the default view.
5
View Options
Here you have the area for your standard view options as well as the specific options provided on the Diffusion Tools tab, see Diffusion Tools Tab.
Polar Heatmap
The polar heatmap illustrates directed diffusions and barriers by using color gradients referring to the number of present molecules in specific angles. On the top you have a visual representation of the Airyscan detector that indicates the fibers that are used for the correlations. These heatmaps are calculated from six pair correlation curves and the pair correlation function (pCF). The pair correlation function is a set of cross-correlations between the central fiber and all fibers located at a given distance from the center. The rainbow pattern is used to map the diagram color to the corresponding correlation amplitude. The color mapping and legend are the same for both heatmaps and the color palette can be selected in the Diffusion Tools tab. A right click on the heatmap displays a menu that allows you to save it as an image on your PC. The displayed degrees can vary based on your system configuration.
Only visible if you have created a second reference image after the spot experiment.
Selects which reference image is displayed int the view.
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Pre-Experiment
Displays the reference image acquired before the experiment.
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Post-Experiment
Displays the reference image acquired after the experiment.
Correlation Chart
Selects which correlation chart and which curves are displayed in the correlation chart and allows you to set the color for the curves. You can select to see the chart for Inner Elements or Outer Elements.
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Curve Binning
Activated: Smoothes the curves by averaging for noisy data.
Amplitude Normalization
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Activate
Normalizes the amplitude to 1.
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Deactivate
Applies no normalization.
Polar Heatmap Palette
Displays and selects the color palette used for the visualization in the polar heatmap.