|
Parameter |
Description |
|
|---|---|---|
|
Reference Image |
Only visible if you have created a second reference image after the spot experiment. |
|
|
– |
Pre-Experiment |
Displays the reference image acquired before the experiment. |
|
– |
Post-Experiment |
Displays the reference image acquired after the experiment. |
|
Display Chart |
Selects which chart is displayed above the correlation chart. |
|
|
– |
Count Rate |
Displays the count rate chart. |
|
– |
Residuals |
Displays the residuals chart. Note that the residuals chart only contains information after a fit was executed for the respective spot. |
|
Filter |
||
|
– |
Detrending |
Activated: Applies a detrending filter to remove the trend (e.g. due to the photo bleaching) from the experiment data. Detrending is calculated by a low-pass filter with the filter time constant provided with the input field. |
|
– |
Dust |
Activated: Applies a dust filter to remove high intensity peaks (caused e.g. by aggregated objects) from the experiment data and sets the parameter with the input field. Dust is considered, if the average intensity multiplied with the parameter value is higher than the mean signal intensity. |
|
Fit Model |
Selects the fit model for the correlation curve. |
|
|
– |
1 Component 2D |
Selects the one component 2D model. |
|
– |
2 Component 2D |
Selects the two component 2D model. |
|
– |
1 Component 3D |
Selects the one component 3D model. |
|
– |
2 Component 3D |
Selects the two component 3D model. |
|
– |
1 Component Anomalous 2D |
Selects the one component anomalous 2D model. |
|
– |
1 Component Anomalous 3D |
Selects the one component anomalous 3D model. |
|
– |
Fit |
Applies the fit model to the currently selected position/curve. |
|
– |
Fit All |
Applies the fit model to all positions/curves. |
|
Amplitude Normalization |
||
|
– |
Activate |
Normalizes the amplitude to 1. |
|
– |
Deactivate |
Applies no normalization. |
|
ωr Calibration |
Selects which ωr should be used for the fit of the curves. |
|
|
– |
Default |
Uses the default setting for the ωr calibration. |
|
– |
Custom |
Opens a browser to select a file with a user generated ωr calibration. This file can be created with the ωr tab. |