This view displays your experiment and the correlation data of the individual measurement positions. The different areas containing the image, the charts and the table can be resized by dragging the individual area boundaries with the pressed left mouse button.
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1 |
Preview Image |
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2 |
Parameter Table |
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3 |
Charts |
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4 |
View Options |
This table displays the data for the different measurement positions. Each position is displayed as a separate row. If a value could not be calculated for a specific position, the corresponding entry in the table is empty. A click on an entry highlights and selects the entire row. You can also select multiple rows when pressing the Ctrl button while selecting the entries. The charts are then updated accordingly. The units for individual parameters are displayed as tooltips. A right click in the table opens a specific context menu, see Parameter Table Context Menu.
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Parameter |
Description |
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Ø |
Activated: Uses this position for the calculation of an average for individual values. The average is displayed on the bottom of the table. |
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Toggles the visibility of the respective spot. |
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ID |
Displays the ID for the individual spot. |
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Color |
Sets the color for the respective measurement point by clicking on the field and selecting a color form the dialog. |
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Name |
Displays and sets the name for the individual position. |
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Count Rate |
Displays the count rate in kHz. |
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CPM |
Displays the CPM in kHz. The color of the value indicates the data quality, see Data Quality Criteria (CPM). |
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Data Quality |
Displays an indication for the fit quality. |
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Number of Molecules |
Displays the number of molecules found at the measurement position. It is calculated by 1/correlation amplitude, where the correlation amplitude is defined as value of the correlation curve at the smallest lag time. |
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Concentration |
Only visible after using a 3D fit model. |
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Surface Concentration |
Only visible after using a 2D fit model. |
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Triplet State Fraction |
Displays the triplet state fraction. One physical property of the utilized fluorophore. Dependent on intensity and if applicable oxygen. A triplet state of e.g. 16% would mean that 16% of all fluorophores are in dark state. |
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Chi2 |
Displays the Chi2 value, see also Fit Calculation. Chi2 is the deviation between the measured FCS curve and the fit curve. A value of 0 would mean a 100% accordance. |
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Diffusion Time |
Displays the diffusion time. |
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Diffusion Coefficient |
Displays the diffusion coefficient. |
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α |
Only visible if you have used an anomalous fit model. |
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Fit Model |
Displays which fit model was used. |
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Date |
Displays the date and time of the experiment. |
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Parameter |
Description |
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Reference Image |
Only visible if you have created a second reference image after the spot experiment. |
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– |
Pre-Experiment |
Displays the reference image acquired before the experiment. |
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Post-Experiment |
Displays the reference image acquired after the experiment. |
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Display Chart |
Selects which chart is displayed above the correlation chart. |
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– |
Count Rate |
Displays the count rate chart. |
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– |
Residuals |
Displays the residuals chart. Note that the residuals chart only contains information after a fit was executed for the respective spot. |
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Filter |
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Detrending |
Activated: Applies a detrending filter to remove the trend (e.g. due to the photo bleaching) from the experiment data. Detrending is calculated by a low-pass filter with the filter time constant provided with the input field. |
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Dust |
Activated: Applies a dust filter to remove high intensity peaks (caused e.g. by aggregated objects) from the experiment data and sets the parameter with the input field. Dust is considered, if the average intensity multiplied with the parameter value is higher than the mean signal intensity. |
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Fit Model |
Selects the fit model for the correlation curve. |
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1 Component 2D |
Selects the one component 2D model. |
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2 Component 2D |
Selects the two component 2D model. |
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– |
1 Component 3D |
Selects the one component 3D model. |
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– |
2 Component 3D |
Selects the two component 3D model. |
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– |
1 Component Anomalous 2D |
Selects the one component anomalous 2D model. |
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1 Component Anomalous 3D |
Selects the one component anomalous 3D model. |
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Fit |
Applies the fit model to the currently selected position/curve. |
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Fit All |
Applies the fit model to all positions/curves. |
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Amplitude Normalization |
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Activate |
Normalizes the amplitude to 1. |
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– |
Deactivate |
Applies no normalization. |
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ωr Calibration |
Selects which ωr should be used for the fit of the curves. |
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Default |
Uses the default setting for the ωr calibration. |
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Custom |
Opens a browser to select a file with a user generated ωr calibration. This file can be created with the ωr tab. |
This tab enables you to create your own custom ωr calculation that you can use for fitting the correlation curves.
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Parameter |
Description |
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Dye |
Selects the dye used for the ωr calibration. |
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Diffusion Coefficient |
Displays and sets the diffusion coefficient for the dye. |
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Calibrate |
Calibrates the ωr value and opens a file browser to save it as a calibration file. |