Top Toolbar

Depending on what you have selected on the left toolbar of the Tiles - Advanced Setup, different features to create tiles or positions are displayed. For information on the various top toolbars, see the list below.

Preview Scan Toolbar

Here you can define the settings for a preview scan. Typically a low magnification objective and a channel which protects your sample (e.g. transmitted light) is used. This will give you a low resolution overview of the sample to mark tile regions and/or positions.

Parameter	Description
Use Experiment Settings	Uses the settings of the experiment for the preview scan.
Channel	Only available if Use Experiment Settings is deactivated. Displays the channels for the preview scan. If you deactivate Use Experiment Settings, you can deselect individual channels here.
Camera Binning	Only available if Use Experiment Settings is deactivated. Sets the binning of the camera for the preview scan experiment only. The exposure time will be compensated automatically for the preview scan. Note that higher binning will reduce the scan time (due to shorter exposures of the camera), but reduce the image resolution.
Delete existing preview images	Activated: Deletes the already existing preview images before the preview scan is started.
Start	Starts the preview scan to acquire the overview image.

Predefined Toolbar

Here you can define the tile regions by means of the number or size.

Parameter	Description
Rectangle	Draws a rectangular tile region.
Circle	Draws a circular tile region
Tiles	Using this mode you have to enter the number of tiles as a reference for the size of the tile region. Enter the number of tiles in the X/Y input fields. If you are adding a circular tile region, enter the number of tiles for the diameter in the Diameter input field.
Size	Using this mode you have to enter the size as a reference for the size of the tile region. Enter the size of the tile region in the X/Y input fields. If you are adding a circular tile region, enter the diameter of the tile region in the Diameter input field.
Top left anchor position	The anchor of the defined shape is at the top left.
Center anchor position	The anchor of the defined shape is centered.
Bottom right anchor position	The anchor of the defined shape is at the bottom right.
Add Tile Region	Adds the tile region to the Tile Regions List and activates it for acquisition. Added tile regions are displayed in the form of red grids in the stage view of the Advanced Tiles Setup.
Selection	Select an element in the stage view to edit or move it.
Add Tile Region	Adds the current tile definition in the image area.
Keep Tool	Activated: Keeps the selected tool active. You can use the tool several times in succession without having to reselect it.

Stake Toolbar

Here you can define the tile regions with stake markers.

Parameter	Description
Add Marker Position	Adds a marker position to the current stage and focus position.
Remove Marker Position	Removes the last marker position.
Rectangle	Draws a rectangular tile region.
Circle	Draws a circular tile region
Polygon	Draws a polygonal tile region
Done	Saves the current tile regions setup and resets the marker positions.

Tiles Carrier Toolbar

Here you can define the tile regions automatically by means of the fill factor of the sample carrier.

A sample carrier must have been selected in the Sample Carrier section of the Tiles tool.
Manually created tile regions and positions (setup by Contour and setup by Predefined) will be deleted if you switch to the setup by Carrier. If you want to combine manual and automatic setup, first use setup by Carrier and then switch to a manual setup. Tile regions that are created automatically by setup by Carrier, are defined to a container and permanently assigned and locked by default, against manual editing. You can unlock the tile regions in the Tiles tool by selecting the desired tile region and unlocking it via the options menu in the tile regions list. You can also unlock all tile regions here if necessary.

Parameter	Description
Select Sample Carrier	Only visible if no sample carrier is currently selected. Opens the dialog to select a sample carrier template.
Create	Creates a tile region for every selected sample carrier container.
Remove	Removes the tile region in every selected sample carrier container.
Fill Factor	Here you can enter the fill factor used to fill the selected container.
Columns/Rows	Here you can add single tile regions to a container by defining the number of columns and rows of the tile. The tile region is always placed at the center of the well container.

Positions Location Toolbar

Here, you can define the positions by means of the location. You can add various positions in the Stage View using the mouse.

Parameter	Description
Selection	Selects an element in the stage view to edit or move it.
Add	Adds a new position on the stage view.
Keep Tool	Activated: Keeps the selected tool active. You can use the tool several times without having to reselect it.
Current X/Y	Displays the current stage position (X/Y).
Add Position	Adds a new position at the current stage position.

Positions Carrier Toolbar

Here you can define the positions automatically on the relevant sample carrier.

A sample carrier must have been selected in the Sample Carrier section of the Tiles tool.
Manually created tile regions and positions (setup by Contour and setup by Predefined) will be deleted if you switch to the setup by Carrier. If you want to combine manual and automatic setup, first use setup by Carrier and then switch to a manual setup. Tile regions that are created automatically by setup by Carrier, are defined to a container and permanently assigned and locked by default, against manual editing. You can unlock the tile regions in the Tiles tool by selecting the desired tile region and unlocking it via the options menu in the tile regions list. You can also unlock all tile regions here if necessary.

Parameter		Description
Select Sample Carrier		Only visible if no sample carrier is currently selected. Opens the dialog to select a sample carrier template.
Create		Creates a position array for every selected container of the sample carrier.
Remove		Removes the position array for every selected container of the sample carrier.
Distribute Positions by
–	Number	Activates the input for Number and Bias.
–	Grid	Activates the input field for an Overlap.
Number		Only visible if Number is selected. Shows the current number of positions that are distributed to newly created position array. Change the number to increase or decrease the number of single positions obtained by a position array.
Bias		Only visible if Number is selected. Adjusts the overall position of the single positions in the position array.
-	None	The single positions will be distributed evenly within the array.
-	Center	The single positions will mainly be distributed near to the center of the position array. Less positions will be at the edges of the array.
-	Edge	The positions will be distributed to the edges of the array. Less positions will be in the center of the array.
Random		Only visible if Number is selected. Activated: The single positions will be distributed randomly within the position array. The overall bias will still be taken into account.
Columns		Only visible if Grid is selected. Sets the number of columns of the position array in each container of the sample carrier.
Rows		Only visible if Grid is selected. Sets the number of rows of the position array in each container of the sample carrier.
Overlap		Only visible if Grid is selected. Shows the current degree of overlap in % of each position in the array relative to its neighbors. Both positive and negative values are possible.

Top Toolbar

See also

ON THIS PAGE