Optionally, you can define the area to be analyzed of each image. In case there are shading effects or other reasons that make you want to include only a certain area of each image for analysis, you can define a frame (rectangle, circle or polygon). Only the area within this frame will be further analyzed.

With the Mode parameter, you can furthermore choose how the analysis treats objects that are cut by the border of the image or the frame:

• Cut at frame: Cuts the objects at the frame.
• Inside only: Discards all objects that are cut by the frame.

Note that the whole ZOI is taken into account for the decision if the object is inside the frame or not (not only the primary object). This means there are cases where the primary object might lie completely within the image/frame, but the Ring or ZOI is cut by the border. If you select Inside only, these objects will be discarded.

Comparison of image analysis results for Cut at frame compared to Inside only: