The following example shows how to use the Zone of Influence (ZOI) method to measure intensities within a ring that is associated to the main object, e.g. the cell nucleus. An application example are transport assays where the intensities of a certain fluorescent marker in the cytoplasm are compared to the intensities within the nucleus.
In this example we use a multichannel image of fluorescence-stained cells. The cell nuclei are stained with AF568 and the mitochondria are stained with AF488. First, we detect the nuclei in the AF568-channel as primary object. A zone of influence is generated around each detected primary object. In this area, we can define a ring and specify its thickness and distance from the main object. You can use this ring to measure intensities or to detect further sub-objects on it. For more information, see Counting the Number of Objects in a Ring around the Nucleus.