In this mode, a new image is automatically added after a defined interval. In the time period between the acquisitions you can set a different focus position. After each recording a new image with extended depth of focus is calculated immediately. After finishing the acquisition, the final image is the one that was calculated last.

  1. You have opened the Manual Extended Depth of Focus tool on the Locate tab.
  1. If you want to store all acquired images in memory for a complete recalculation of the EDF image after each single acquisition, activate Z-Stack. Note that the more images you acquire, the more time it takes to calculate the EDF.
  2. Click Timer.
  3. Adjust the length of your interval with the Interval slider or input field. Set the interval long enough so that you can comfortably move the specimen to a new focus position. After the time elapses, an image is automatically acquired at your current position.
  4. Click Start to acquire the images.
  5. The Central Screen Area is split into two parts. On the left you can see the current live image, on the right you see the currently calculated extended focus image.
  6. Move to a new focus position after each acquisition.
  7. Repeat these steps until you have sharp images from all desired areas of your sample.
  8. Click Stop to finish your acquisition. If you want to pause the acquisition, click Pause.
  1. You have finished the acquisition of the manual EDF image. The resulting image is displayed in the Central Screen Area.