1. You have set up the system. For more information, see Acquiring Images - Best Practice.
  2. The Extended Camera tool is selected, see Extended Camera Tool.
  1. To perform the Shading Correction, in the Extended Camera tool, in the section Model Specific, select Raw Image in the Polarization Channel Live, open the section Post Processing and perform the shading correction, for example on a plain filter membrane. To do so, slightly defocus the membrane until the surface texture disappears.
  2. Click Define.
  3. Inspect the results of Shading Correction by switching between the channels using Display Channels.
  4. The selected channel shows a comparable image brightness of the membrane filter for all selected polarization channels. Note that a shading effect has an impact on the image analysis results due to an inhomogeneous brightness distribution. The shading correction shall be applied to generate a homogeneous illuminated image as base for a successful automated image analysis.
  1. You have applied and verified the Shading Correction.
  2. In the next step, you define an image acquisition profile.