Correlative sample holders have three fiducial markers enabling a three point calibration (signed with the numbers 1-2-3) The calibration markers consist of one small (length 50 µm) and a large L-shape marker (length 1 mm). The bigger marker is used for coarse orientation, whereas the smaller marker is used for the calibration.
Calibrating the Sample Holder
Setting Calibration Options
In step 1 of the wizard, the following options should be activated to follow our recommended workflow:
- Check if the Automatic movement to next marker checkbox is activated.
- This will automatically move the stage to the next marker position after you have confirmed the position of the marker and clicked on Next.
- Check if the Use automatic marker detection checkbox is activated.
- The software will try to find the correct positions of each marker automatically.
- If you need to change the marker color, or check if the marker orientation is set correctly, activate the Use settings for marker detection checkbox to access these functions.
- Click on Next to move to the next wizard step.